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anti p 4ebp1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti p 4ebp1
    Anti P 4ebp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+4e+bp1/Phospho-4E-BP1+(Thr37%2F46)+Antibody/10__1038_slash_s41420___026___03349___8-194-30-33
    Average 96 stars, based on 1248 article reviews
    anti p 4ebp1 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: Neurons with granulovacuolar degeneration bodies are resilient to tau-induced protein synthesis impairment
    Article Snippet: 4E-BP1 , , Rabbit monoclonal , , 1:1000 , Cell Signaling Technology , 9644. .. p-4E-BP1 , Thr 37 /Thr 46 , Rabbit monoclonal , , 1:1000 , Cell Signaling Technology , 2855. ..

    Article Title: Amino acid restriction sensitizes lung cancer cells to ferroptosis via GCN2-dependent activation of the integrated stress response
    Article Snippet: Band intensities were quantified with the ImageJ software. .. Primary antibodies used were 4E-BP1 (9452; Cell signaling technology (CST)), ATF4 (11815; CST), α-Tubulin (T6199; Sigma-Aldrich), CHAC1 (15207-1-AP; Proteintech), CHOP (15204-1; Proteintech), eIF2α (5324; CST), Ferritin (ab75973-1001; Abcam), GADD34 (10449-1-AP; Proteintech), GAPDH (5174; CST), GCN2 (3302; CST), GCLC (ab41463; Abcam), LC3B (NB100–2220SS; Novusbio), p-4E-BP1 (2855; CST), p-eIF2α Ser 51 (3398; CST), p-GCN2 (ab75836; Abcam), p-S6 (2211; CST), S6 (2217; CST), TfrC (13–6800; Invitrogen), p-PKR (ab32036; Abcam), p-PERK (ab192591; Abcam), PERK (3192; CST), PKR (sc-6282; SCBT), and Hsp90 (4877; CST). .. Secondary antibody used was peroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG (H + L) (111-035-003; Jackson Immunoresearch) and peroxidase-conjugated AffiniPure Goat Anti-Mouse IgG (H + L) (115-035-003; Jackson Immunoresearch).

    Article Title: Effects of branched-chain amino acids on iron deficiency-induced muscle atrophy
    Article Snippet: p70 S6 Kinase , Rabbit , 9202 , 1:1000 , Cell Signaling Technology. .. p-4E-BP1 , Rabbit , 9451 , 1:1000 , Cell Signaling Technology. .. 4E-BP1 , Rabbit , 9452 , 1:1000 , Cell Signaling Technology.

    Nucleic Acid Electrophoresis:

    Article Title: The redundant protein synthesis gene Aimp1 challenges the canonical inverse relationship between translation and autophagy.
    Article Snippet: .. Other TEPM were treated with Torin (250nM, Adooq Bioscience #A11587) for 2 hours followed by + BafA1 (50nM) for 2 hours with gel electrophoresis of equal protein concentrations followed by immunoblotting with primary antibodies against LC3-II (1:1000, Cell Signaling Technologies, D11, #3868), LAMP1 (1:1000, Santa Cruz Biotechnology, sc-20011, Clone H4A3), pRPS6 (1:1000, Cell Signaling Technologies, #4858), RPS6 (1:1000, Cell Signaling Technologies, #2217),pSTAT3 (Y705) (1:1000, Cell Signaling Technologies, #9145), STAT3 (1:1000, Cell Signaling Technologies, #9139), p-4E-BP1 (1:1000, Cell Signaling Technologies, #28455), 4EBP1 (1:1000, Cell Signaling Technologies, #9644), or Actin (1:10,000, Cell Signaling Technologies, 8H10D10, #3700) diluted in 5% (w/v) milk in TBS-T. .. Other TEPM were treated with Torin (250nM, Adooq Bioscience #A11587) for 2 hours followed by + BafA1 (50nM) for 2 hours with gel electrophoresis of equal protein concentrations followed by immunoblotting with primary antibodies against LC3-II (1:1000, Cell Signaling Technologies, D11, #3868), LAMP1 (1:1000, Santa Cruz Biotechnology, sc-20011, Clone H4A3), pRPS6 (1:1000, Cell Signaling Technologies, #4858), RPS6 (1:1000, Cell Signaling Technologies, #2217),pSTAT3 (Y705) (1:1000, Cell Signaling Technologies, #9145), STAT3 (1:1000, Cell Signaling Technologies, #9139), p-4E-BP1 (1:1000, Cell Signaling Technologies, #28455), 4EBP1 (1:1000, Cell Signaling Technologies, #9644), or Actin (1:10,000, Cell Signaling Technologies, 8H10D10, #3700) diluted in 5% (w/v) milk in TBS-T.

    Western Blot:

    Article Title: The redundant protein synthesis gene Aimp1 challenges the canonical inverse relationship between translation and autophagy.
    Article Snippet: .. Other TEPM were treated with Torin (250nM, Adooq Bioscience #A11587) for 2 hours followed by + BafA1 (50nM) for 2 hours with gel electrophoresis of equal protein concentrations followed by immunoblotting with primary antibodies against LC3-II (1:1000, Cell Signaling Technologies, D11, #3868), LAMP1 (1:1000, Santa Cruz Biotechnology, sc-20011, Clone H4A3), pRPS6 (1:1000, Cell Signaling Technologies, #4858), RPS6 (1:1000, Cell Signaling Technologies, #2217),pSTAT3 (Y705) (1:1000, Cell Signaling Technologies, #9145), STAT3 (1:1000, Cell Signaling Technologies, #9139), p-4E-BP1 (1:1000, Cell Signaling Technologies, #28455), 4EBP1 (1:1000, Cell Signaling Technologies, #9644), or Actin (1:10,000, Cell Signaling Technologies, 8H10D10, #3700) diluted in 5% (w/v) milk in TBS-T. .. Other TEPM were treated with Torin (250nM, Adooq Bioscience #A11587) for 2 hours followed by + BafA1 (50nM) for 2 hours with gel electrophoresis of equal protein concentrations followed by immunoblotting with primary antibodies against LC3-II (1:1000, Cell Signaling Technologies, D11, #3868), LAMP1 (1:1000, Santa Cruz Biotechnology, sc-20011, Clone H4A3), pRPS6 (1:1000, Cell Signaling Technologies, #4858), RPS6 (1:1000, Cell Signaling Technologies, #2217),pSTAT3 (Y705) (1:1000, Cell Signaling Technologies, #9145), STAT3 (1:1000, Cell Signaling Technologies, #9139), p-4E-BP1 (1:1000, Cell Signaling Technologies, #28455), 4EBP1 (1:1000, Cell Signaling Technologies, #9644), or Actin (1:10,000, Cell Signaling Technologies, 8H10D10, #3700) diluted in 5% (w/v) milk in TBS-T.

    Article Title: Composition for alleviating, preventing or treating sarcopenia, containing whey protein hydrolysate as active ingredient
    Article Snippet: .. Western blot was conducted in the same manner as in Test Example 5 using the gastrocnemius taken in Test Example 3. p-PI3K antibody was purchased from Abcam (England); PI3K, p-Akt, Akt, p-S6K1, S6K1, p-4E-BP1 and 4E-BP1 antibodies were purchased from Cell Signaling Technology (Danvers, USA); and Gapdh antibody was purchased from GeneTex (California, USA). ..

    Pyrolysis Gas Chromatography:

    Article Title: Limosilactobacillus reuteri ATG-F4 Ameliorates Dexamethasone-Induced Muscle Atrophy through Modulation of Gut Microbiota.
    Article Snippet: Membranes were blocked overnight at 4°C in SuperBlock (PBS) blocking buffer (pH 7.4) containing Kathon antimicrobial agent and incubated with primary antibodies (all from Cell Signaling Technology, USA) at a dilution of 1:1000. .. The following primary antibodies were used: myostatin, p-Akt, Akt, p-FOXO3a, FOXO3a, Atrogin-1, MuRF1, p-AMPK, AMPK, PGC-1α, mtTFA, p-p70S6K, p70S6K, p-rpS6, rpS6, p-4E-BP1, 4E-BP1, and β-actin as a loading control (1:1000, Cell Signaling Technology, USA). .. Blots were washed four times with 0.1% Tween-20 in TBS (TBS-T) before incubation for 1 h at approximately 22–25°C with a goat anti-rabbit IgG HRP-conjugated secondary antibody (Bio-Rad) in TBS-T containing 3% BSA (Bovogen, USA).

    Control:

    Article Title: Limosilactobacillus reuteri ATG-F4 Ameliorates Dexamethasone-Induced Muscle Atrophy through Modulation of Gut Microbiota.
    Article Snippet: Membranes were blocked overnight at 4°C in SuperBlock (PBS) blocking buffer (pH 7.4) containing Kathon antimicrobial agent and incubated with primary antibodies (all from Cell Signaling Technology, USA) at a dilution of 1:1000. .. The following primary antibodies were used: myostatin, p-Akt, Akt, p-FOXO3a, FOXO3a, Atrogin-1, MuRF1, p-AMPK, AMPK, PGC-1α, mtTFA, p-p70S6K, p70S6K, p-rpS6, rpS6, p-4E-BP1, 4E-BP1, and β-actin as a loading control (1:1000, Cell Signaling Technology, USA). .. Blots were washed four times with 0.1% Tween-20 in TBS (TBS-T) before incubation for 1 h at approximately 22–25°C with a goat anti-rabbit IgG HRP-conjugated secondary antibody (Bio-Rad) in TBS-T containing 3% BSA (Bovogen, USA).



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